@inproceedings{AholaSchabelMuelleretal.2018, author = {Ahola, Marja Birgit and Schabel, Samuel and M{\"u}ller, Tim M. and Altherr, Lena C. and Pelz, Peter}, title = {Optimizing Pressure Screen Systems in Paper Recycling - Optimal System Layout, Component Selection and Operation}, series = {EngOpt 2018 Proceedings of the 6th International Conference on Engineering Optimization}, booktitle = {EngOpt 2018 Proceedings of the 6th International Conference on Engineering Optimization}, publisher = {Springer}, year = {2018}, language = {en} } @inproceedings{AholaSchabelMuelleretal.2018, author = {Ahola, Marja Birgit and Schabel, Samuel and M{\"u}ller, Tim M. and Altherr, Lena C. and Pelz, Peter}, title = {Multi-Criteria Optimization of Pressure Screen Systems in Paper Recycling - Balancing Quality, Yield, Energy Consumption and System Complexity}, series = {EngOpt 2018 Proceedings of the 6th International Conference on Engineering Optimization}, booktitle = {EngOpt 2018 Proceedings of the 6th International Conference on Engineering Optimization}, editor = {Rodrigues, H. C.}, publisher = {Springer}, year = {2018}, language = {en} } @article{FerozFerlezLefoulonetal.2018, author = {Feroz, Hasin and Ferlez, Bryan and Lefoulon, Cecile and Ren, Tingwei and Baker, Carol S. and Gajewski, John P. and Lugar, Daniel J. and Gaudana, Sandeep B. and Butler, Peter J. and H{\"u}hn, Jonas and Lamping, Matthias and Parak, Wolfgang J. and Hibberd, Julian M. and Kerfeld, Cheryl A. and Smirnoff, Nicholas and Blatt, Michael R. and Golbeck, John H. and Kumar, Manish}, title = {Light-Driven Chloride Transport Kinetics of Halorhodopsin}, series = {Biophysical Journal}, volume = {115}, journal = {Biophysical Journal}, number = {2}, issn = {1542-0086}, doi = {10.1016/j.bpj.2018.06.009}, pages = {353 -- 360}, year = {2018}, abstract = {Despite growing interest in light-driven ion pumps for use in optogenetics, current estimates of their transport rates span two orders of magnitude due to challenges in measuring slow transport processes and determining protein concentration and/or orientation in membranes in vitro. In this study, we report, to our knowledge, the first direct quantitative measurement of light-driven Cl- transport rates of the anion pump halorohodopsin from Natronomonas pharaonis (NpHR). We used light-interfaced voltage clamp measurements on NpHR-expressing oocytes to obtain a transport rate of 219 (± 98) Cl-/protein/s for a photon flux of 630 photons/protein/s. The measurement is consistent with the literature-reported quantum efficiency of ∼30\% for NpHR, i.e., 0.3 isomerizations per photon absorbed. To reconcile our measurements with an earlier-reported 20 ms rate-limiting step, or 35 turnovers/protein/s, we conducted, to our knowledge, novel consecutive single-turnover flash experiments that demonstrate that under continuous illumination, NpHR bypasses this step in the photocycle.}, language = {en} }